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视黄醇结合蛋白4与脂肪酸合成酶及肝脂肪酶的相关研究
Relationship between Retinol binding protein 4 and fatty acid synthase and hepatic lipase

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DOI:
作者:
邱慧玲，方启晨，吴海娅，陆俊茜，包玉倩
QIU Huiling, FANG Qichen, WU Haiya, LU Junxi, BAO
作者单位:
上海交通大学附属第六人民医院内分泌代谢科、上海市糖尿病临床医学中心、上海市糖尿病研究所
Department of Endocrinology and Metabolism, Shanghai Jiao Tong University Affiliated Sixth People´s Hospital; Shanghai Clinical Center for Diabetes
关键词:
视黄醇结合蛋白4；甘油三酯；脂肪酸合成酶；肝脂肪酶
Retinol binding protein 4; Triglyceride; Fatty acid synthase; Hepatic lipase
摘要:
目的 研究视黄醇结合蛋白4（RBP4）对脂肪酸合成酶及肝脂肪酶的影响。方法 用0ng/ml、10ng/ml、100ng/ml及1000ng/ml不同RBP4浓度培养基干预HepG2细胞，Realtime-PCR检测脂肪酸合成酶（FAS）及肝脂肪酶（HL）基因mRNA表达水平，分光光度法分析FAS及HL活性。结果 1. 不同浓度RBP4处理12h见到：100ng/ml RBP4处理组HepG2细胞FAS基因mRNA表达较对照组有升高趋势（P=0.087），100ng/ml RBP4浓度培养12h较培养1h明显升高（P<0.05），培养24h较培养1h有升高趋势（p=0.098）。 2. 不同RBP4浓度刺激HepG2细胞10min后结果见到，1000ng/ml处理组较对照组、10ng/ml及100ng/ml处理组HL基因mRNA水平显著升高（P值<0.01或<0.05），20min后1000ng/ml处理组及10ng/ml处理组较对照组升高，而30min后无明显差异。3. HepG2细胞体外培养给予不同浓度RBP4孵育24h后，与对照组相比，10ng/ml、1000ng/ml RBP4组的FAS酶活性显著上升（p<0.05），随着RBP4浓度升高FAS酶活性呈增高趋势；而HL酶活性无明显变化。 结论 1. 100ng/ml的RBP4能增加FAS基因mRNA的表达，RBP4可能影响FAS酶活性，FAS酶活性随着RBP4浓度的增加有增强趋势。 2. 高浓度RBP4短时间干预可以增加HepG2细胞HL基因mRNA的表达，未见到RBP4对HL酶活性的影响。
objective The study was to investigate the relationship between serum RBP4 and fatty acid synthase (FAS) and hepatic lipase (HL). Methods HepG2 cells were cultured with the following concentrations of RBP4 in medium (control group, 10ng/ml, 100ng/ml and 1000ng/ml). Fluorescent realtime RT-PCR was used to quantitated the mRNA expression amount of FAS and HL genes in these grouped HepG2 cells. The activity of the above enzymes was measured by spectrophotometric. Results 1. The expression of FAS mRNA was higher than that of control group when HepG2 cells cultured with 100ng/ml RBP4 in medium for 12h. In addition, we found 100ng/ml RBP4 concentration of culture for 12h was significantly higher than that of 1h (P<0.05). 2. The expression of HL mRNA was tend to increase more than that of control group when HepG2 cells were cultured with 1000ng/ml RBP4 in medium for 10min and 20min (P values <0.01 or <0.05). But no significant differences were found among the four RBP4 concentrations cultured for 30min. 3. When cultured with different RBP4 concentrations in medium for 24h, the activity of FAS significantly increased in 10ng/ml and 1000ng/ml RBP4 group than that of control group (p<0.05). FAS activity showed an increasing trend as RBP4 concentration increased. No significantly difference was found in HL activity. Conclusions 1. RBP4 concentration of 100ng/ml could increase the mRNA expression of FAS, it might impact on FAS activity. FAS activity showed an increasing trend as RBP4 concentration increased. 2. High concentrations of RBP4 intervention on HepG2 cells for 10min or 20min could increase the mRNA expression of HL. No significant differences were found in HL activity.

 

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