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高脂饮食加重压力超负荷诱发的小鼠心肌肥厚(重投）
High fat diet increased pressure overload-induced cardiac hypertrophy

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DOI:
作者:
林利 葛静怡 刘明 葛均波 邹云增
LIN Li,GE Jingyi,LIU Ming,GE Jun-bo,ZOU Yun-zeng
作者单位:
200120 上海，同济大学附属东方医院心内科； 200032上海，复旦大学生物医学研究院； 200032上海，复旦大学附属中山医院 上海市心血管病研究所
1.Cardiology Department, Shanghai East Hospital，Tongji University, Shanghai 200120, China. 2. Research Center for Cardiovascular Diseases, Institutes of Biomedical Sciences, Fudan University, Shanghai, 200032，China 3.Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital，Fudan University, Shanghai 200032, China
关键词:
心肌肥厚；压力超负荷；AT1
Cardiac hypertrophy; Pressure overload; AT1
摘要:
目的：探讨高脂饮食能否加重压力超负荷诱发的ApoE基因敲除小鼠心肌肥厚。方法：通过缩窄升主动脉（TAC）构建ApoE基因敲除小鼠压力超负荷心肌肥厚模型。8-10周龄ApoE基因敲除小鼠随机分为3组：假手术组，TAC组和高脂饮食+TAC组。TAC 两周后，对小鼠心脏进行超声及病理形态学检测，同时测量左心室压力、全心/体重比值以及相关肥厚基因及蛋白。结果：相对于假手术组，TAC组心肌肥厚各项指标值明显升高（P<0.05）。高脂饮食+TAC组心肌肥厚的各项指标值均显著高于TAC组（P<0.05）；另外，TAC组血管紧张素Ⅱ受体（AT1）较假手术组明显升高，而高脂饮食+TAC组的AT1较TAC组也显著升高。结论：高脂饮食加重压力超负荷诱发的小鼠心肌肥厚可能与AT1有关。
Objective This study is designated to investigate whether high fat diet increase cardiac hypertrophy induced by pressure overload in ApoE-deficient mice. Methods Constricting transverse aorta (TAC) were used as a model for pressure-overload cardiac hypertrophy. ApoE -deficient (ApoE KO) mice of 8-10 weeks were divided into three groups，respectively :Sham-operated group (Sham), TAC group (TAC) and TAC plus high fat diet group (HFD+TAC). After 2 weeks of TAC, echocardiography assessments were performed for the geometry and function of hearts, and then mice were subjected to catheterization to measure the left ventricular pressure. Cardiac hypertrophy was confirmed further by histological analysis. The expressions of special genes and protein associated with cardiac hypertrophy were determined by Real-time PCR or Western blotting. Results Compared to Sham group, all the values of cardiac hypertrophic index for TAC group increased significantly (P<0.05). Compared with TAC group,High fat diet can markedly increase cardiac hypertrophy and significantly increase the upregulation of Angiotensin II receptor type 1 (AT1 receptor) induced by pressure overload. Conclusions High fat diet can increase pressure overload-induced cardiac hypertrophy, and the underlying mechanisms may be that high fat diet increase the expression of AT1 receptor.

 

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