注册本刊作者作者投稿查稿
专家远程审稿编辑在线审稿
编务办公专区主编办公专区
下载文档
工作动态
联系方式
发行周期:
月刊
主管单位:
上海市卫生健康委员会
主办单位:
上海市医学会
编辑出版:
《上海医学》编辑部
联系地址:
上海市北京西路1623号
邮编:
200040
电话:
021-62178606
传真:
021-62178606
邮箱:
smasmj@shsma.org.cn
ISSN:
ISSN0253-9934
CN:
CN31-1366/R
收款账号:
1001255309008900719
账户名:
上海市医学会
开户行:
工商银行上海市静安寺支行
友情链接

当前位置：首页 > 过刊浏览->2011年第7期

双向凝胶电泳进行肾癌与健康人血清中差异表达蛋白的筛选和鉴定

浏览(2952) 下载(0)

DOI:
作者:
石农，徐罡，袁海川，项翠琴，陆晔，温晖，顾斌，刘俊，马志承，张立旻，丁强，张元芳
Nong Shi, Gang Xu, Haichuan Yuan, Cuiqin Xiang……
作者单位:
复旦大学附属华山医院宝山仁和分院泌尿外科 复旦大学附属华山医院泌尿外科 上海市疾病预防控制中心
Department of Urology, Huashan Hospital Baoshan Renhe Branch, Fudan University Department of Urology，Huashan Hospital, Fudan University Shanghai Municipal Center for Disease Control & Prevention
关键词:
肾细胞癌；血清标志物；双向凝胶电泳
Renal cell carcinoma; Serum biomarkers; Two-dimensional gel electrophoresis
摘要:
摘要 目的：利用双向凝胶电泳（two dimensional gel electrophoresis，2-DE）结合基质辅助激光解析电离化/飞行时间质谱（matrix assisted laser desorption ionization/time of flight mass spectrometry，MALDI-TOF-MS）技术对小肾癌和健康人的血清蛋白进行差异蛋白质的筛选和鉴定，以寻找潜在的肾癌肿瘤标记物。方法：20例小肾癌和20例健康人中各抽取少量静脉血，并分离出血清，利用2-DE结合MALDI-TOF-MS技术分析血清蛋白质表达谱的测定，并用Mascot软件搜索NCBInr数据库，进行质谱学的鉴定。结果：在凝胶图像上，总共发现了27个差异蛋白质位点。我们进一步对其中的5个位点进行了切胶、酶解和质谱鉴定，最后分别发现是凝血因子XIII，补体C3C，C3前体，血液结合素和alpha-1-B-糖蛋白，其中部分血清蛋白质是已知的恶性肿瘤发生发展中的调节因子。结论：我们利用2-DE技术鉴定了5个在小肾癌患者和健康人血清中的差异表达蛋白质，这些蛋白质有可能在肾癌的早期诊断中起一定的肿瘤标记物的作用。
Abstract: Purpose To identify the proteomic differences between renal cell carcinoma (RCC) and benign renal tumors. Methods we compared the serum protein profiles of patients with small RCC to those of healthy individuals to identify the differentially expressed proteins. Serum samples were collected from 20 patients with small RCC and 20 healthy individuals. The serum protein expression profiles were analyzed by two-dimensional (2-D) gel electrophoresis and matrix assisted laser desorption ionization/time of flight mass spectrometry (MALDI-TOF-MS). Results Twenty-seven proteins with differences in expression levels between small RCC patients and healthy volunteers were identified. Five differentially expressed proteins identified by using mass spectrometry included coagulation factor XIII B, complement C3 and its precursor, hemopexin, and alpha-1-B-glycoprotein. Some of these serum proteins are known regulators of tumor progression in human malignancies. Conclusion we successfully applied 2-D gel electrophoresis and identified five serum proteins differentially expressed between patients with small RCC and healthy volunteers. These proteins may provide novel biomarkers for early detection and diagnosis of human RCC.

 

主管单位：上海市卫生健康委员会  主办单位：上海市医学会
©2025《上海医学》编辑部 沪ICP备09078599号
您是本站第29250311名访问者
网站管理