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吗啡成瘾时脑细胞凋亡数目的增加
Evidence for morphine-induced neuronal apoptosis in rat brain

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DOI:
作者:
刘立伟, 王新华, 傅强, 吴青华，傅舒昆
LIU Li-wei,WANG Xin-hua,FU Qiang,Wu Qing-hua,FU Sk
作者单位:
同济大学附属第十人民医院麻醉科 同济大学附属东方医院麻醉科 解放军总医院麻醉科 解放军第一五零医院麻醉科 同济大学附属第十人民医院麻醉科
Department of Anesthesiology, Tenth People's Hospital of Tongji University Department of Anesthesiology, East Hospital of Tongji University Department of Anesthesiology, General Hospital of PLA
关键词:
吗啡；鼠脑；细胞凋亡
morphine; rat brain; apoptosis
摘要:
目的 观察吗啡成瘾大鼠脑细胞凋亡数目的变化。方法 24只体重为190～210kg的成年SD大鼠随机分为3组：吗啡依赖组、吗啡戒断组和生理盐水对照组，每组8只。依据药物递增原则，依赖组和戒断组大鼠腹腔内给予吗啡13天，建立吗啡成瘾模型。戒断组大鼠在成瘾后腹腔内注射纳洛酮5mg/kg，诱导戒断30分钟。对照组大鼠在相同的治疗时间腹腔内注射0.9%生理盐水。凋亡细胞通过免疫组织化学方法检测。结果 与对照组比较，吗啡成瘾时脑细胞凋亡数目明显增多（P﹤0.01），同样，与对照组比较，纳洛酮诱发戒断可明显增加凋亡性神经元的数目（P﹤0.01）。结论 长期应用吗啡可诱发大鼠脑细胞异常凋亡，这可能是阿片类药物引起脑内神经改变的机制之一。
Objective To determine whether morphine has an effect on apoptosis of rat brain cells in vivo. Methods Twenty-four adult male Sprague-Dawley rats were randomly divided into 3 groups（n=8）: chronic morphine-dependent group, chronic morphine-abstinent group and control group. The rats in dependent group and abstinent group were chronically treated with morphine for 13 days till morphine dependent models were founded. Rats in abstinent group were induced withdrawal syndromes with naloxone 5mg/kg for 30 minutes after chronic morphine. The rats in control group were injected with saline. Apoptosis cells were enumerated by the technique of immunohistochemistry. Results Chronic treatment with morphine, compared with saline solution administration, induced marked neuronal apoptosis in rat brain (P﹤0.01). Similarly, final naloxone injection in morphine- dependent rats significantly increased the number of apoptotic cells in rat brain compared to those injected with saline (P﹤0.01). Conclusion The results of these in vivo studies demonstrate that chronic morphine can promote abnormal neuronal apoptosis in rat brain, suggesting that morphine-induced neuronal apoptosis might have a relevant role in opiate-induced neuronal damage.

 

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