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异氟烷后处理减轻大鼠盲肠结扎穿孔所致急性肺损伤
Postconditioning with isoflurane reduces acute lung injury induced by cecal ligation and puncture in rats

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DOI:
作者:
吕翔
LV Xiang
作者单位:
上海交通大学医学院附属第九人民医院
Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine
关键词:
异氟烷；急性肺损伤；盲肠结扎穿孔；后处理; NF-KappaB
Isoflurane; Acute lung injury; Cecal ligation and puncture; Postconditioning; NF-KappaB
摘要:
摘要：目的 研究异氟烷( Isoflurane,ISO)后处理对大鼠盲肠结扎穿孔（Cecal ligation and puncture ,CLP）所致急性肺损伤(Acute lung injury ,ALI)的保护作用。方法 48只SD雄性大鼠随机分为4组(每组n= 12)，分别为正常组（N组），未行任何处理；假手术组(Sham组)，单纯行开腹关腹术；盲肠结扎穿孔术组( CLP组)，行CLP建立大鼠ALI模型；异氟烷组( ISO 组)，建立CLP模型后4 h使大鼠吸入1.4%（1MAC）异氟烷2h，每组分别在术后6和24小时随机处死六只大鼠作为Ⅰ、Ⅱ亚组。心脏采血, 取上清液用ELISA法测定肿瘤坏死因子α(TNF-α) 和白细胞介素6( IL- 6) 的含量。取左肺测定肺湿/干比(W /D)、右肺HE染色观察肺组织病理学改变、免疫组化法观察NF-KappaB的表达。结果 肺组织病理学检测结果显示：CLP组大鼠肺组织损伤严重, ISO组肺组织损伤较轻。与N组比较CLP组及ISO组炎性因子TNF-α、IL- 6及核转录因子NF-KappaB的表达均升高，但ISO组较CLP组降低。结论 异氟烷后处理对脓毒症急性肺损伤具有保护作用,这种保护作用可能部分是通过抑制炎性因子和NF-KappaB的表达来实现的。
【Abstract】 PURPOSE： To study the protective effects of isoflurane postreatment on acute lung injury induced by cecal ligation and puncture in rats. METHODS：48 male Sprague-Dawley rats were randomly assigned to 4 groups(n=12 each): normal(N) group,without any treatment;Sham-operated control(Sham) group; with incision and closure of the abdomen only; cecal ligation and puncture(CLP) group, using the CLP model to induce the acute lung injury; isoflurane(ISO) group, rats were anesthetized with 1.4%(1MAC) isoflurane for an additional 2h starting at 4h after CLP model established. Then 6 rats in each group were randomly killed by decapitation to establish theⅠ、Ⅱ subgroup.We monitored the tumor necrosis factor-α(TNF-α) 、IL-6 levels with the ELISA method. The extent of ALI was evaluated by lung wet/dry ratio and histologic examination.In addition, the expression of the NF-KappaB were also determined. RESULTS：The histopathology results showed that the rats in CLP group had more serious lung injure compared with ISO group, the TNF-α 、IL-6 levels and the expression of the NF-KappaB of the rats in CLP and ISO groups were higher than in the N and Sham groups while they were lower in the ISO group compared with the CLP group. CONCLUSION：Postconditioning with isoflurane can attenuate acute lung injury induced by cecal ligation and puncture in rats. The anti-inflammatory protection seems to be mediated partly through inhibition of the pro-inflammatory factors and the expression of the NF-KappaB.

 

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