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背根节8型代谢型谷氨酸受体对神经病理性痛的影响
The role of dorsal root ganglion metabotropic glutamate receptor 8 in the neuropathic pain

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DOI:
作者:
王华，陈锐，周全红，江伟
Wang Hua, Chen Rui, Zhou Quanhong, Jiang Wei
作者单位:
上海交通大学附属上海第六人民医院麻醉科
Department of Anesthesiology, Shanghai Sixth people’s Hospital, Shanghai Jiaotong University
关键词:
8型代谢型谷氨酸受体；背根节；神经病理性痛
Metabotropic glutamate receptor 8; Dorsal root ganglion; Neuropathic pain
摘要:
【摘要】 目的 探讨背根节8型代谢型谷氨酸受体对神经病理性痛的影响。方法 取雄性Sprague-Dawley大鼠60只，随机分入假手术组和神经病理性痛模型组，术后第7天模型确立后将两组各分为三个亚组，分别鞘内注射0.9％氯化钠，DCPG和MSOP+DCPG，用von frey丝测定大鼠给药后的缩足反应阈值，用western blot检测脊髓和背根节8型代谢型谷氨酸受体的表达。结果 各组间基础痛阈无差异（P >0.05），术后7天模型组缩足反应阈值较术前显著降低（P <0.01），假手术组则未见差异（P >0.05）。模型组鞘内注射DCPG后与鞘内注射0.9％氯化钠比较缩足反应阈值显著提高（P <0.05）,但鞘内注射MSOP+DCPG与鞘内注射0.9％氯化钠比较差异无统计学意义（P >0.05）。假手术组鞘内注射DCPG和MSOP+DCPG后缩足反应阈值较注射0.9％氯化钠无显著差异（P >0.05）。western blot结果显示，模型组背根节8型代谢型谷氨酸受体表达明显低于假手术组（P <0.05），而两组脊髓内均未检测到8型代谢型谷氨酸受体的表达。结论 激活背根节8型代谢型谷氨酸受体可以缓解神经病理性痛大鼠的痛觉过敏，神经病理性痛形成过程中背根节8型代谢型谷氨酸受体表达下调。
【Abstract】 Objective To investigate the role of dorsal root ganglion (DRG) metabotropic glutamate receptor 8(mGluR8) in the neuropathic pain. Methods Sixty male Sprague-Dawley rats were randomized into neuropathic modle group and sham operation group. The reliability of the model was certified at day 7 after operation. Then each group was divided into 3 subgroups, intrathecal administration of normal saline, DCPG and MSOP+DCPG separately. Paw withdrawal threshold was assessed by Von Frey test. The expressions of mGluR8 both in the spinal cord and dorsal root ganglion were evaluated by western blotting. Result No statistical difference of basic pain threshold had been detected among all groups (p>0.05). At day 7 after operation，paw withdrawal threshold was significantly decreased in neuropathic model group (p<0.01), whereas no change was found in sham operation group (p>0.05). In the neuropathic pain model group, the intrathecal injection of DCPG significantly increased the paw withdrawal threshold compared with the intrathecal injection of normal saline (p<0.05), but the intrathecal injection of MSOP+DCPG produced no effect (p<0.05). In the sham operation group, the intrathecal injection of DCPG or MSOP+DCPG failed to induce any effect (p>0.05). Decreased mGluR8 expression in DRG was observed in model group by western blotting in comparison with sham group (p<0.05), while no mGluR8 was found in rat spinal cord. Conclusion Activation of DRG mGluR8 attenuated the hyperalgesia in neuropathic pain model rats. The expression of DRG mGluR8 significantly decreased in neuropathic model rats.

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