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2型糖尿病家系脂联素基因多态性与血清高敏C反应蛋白的相关性研究
The association of adiponectin gene polymorphism with high sensitive C-reactive protein in type 2 diabetes families.

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DOI:
作者:
梁松
Liang Song
作者单位:
成都市第二人民医院
The Second people’s hospital of Chengdu city
关键词:
2型糖尿病，一级亲属，高敏C反应蛋白，脂联素，基因多态性
type 2 diabetes mellitus, first degree relatives, high sensitive C reactive protein, adiponectin , gene polymorphism
摘要:
目的 探讨2型糖尿病(T2DM)家系中T2DM患者及糖代谢正常一级亲属脂联素基因多态性与血清高敏C反应蛋白(hs-CRP)的相关性。方法 (1) 选取重庆地区汉族人群中符合入选条件的T2DM家系。总共纳入553名研究对象，有251名T2DM患者，175名糖代谢正常(NGT)一级亲属，以及127名入选家系成员的配偶。研究对象测定人体测量学参数、血压、肾功、血脂谱、hs-CRP等，行口服葡萄糖耐量试验(OGTT)。采用WHO标准诊断T2DM。(2) 去除家系中的二级亲属、糖调节受损(IGR)一级亲属、配偶中有DM家族史和糖代谢异常者（包括患有T2DM及IGR者）。(3)以聚合酶链式反应-限制性内切酶长度多态性(PCR-RFLP)技术，对研究对象脂联素基因外显子2 Glyl5Gly位点进行基因分型。分别比较各组人体测量参数、血压、血糖、血脂、胰岛素、肾功、hs-CRP等临床和生化指标的差别，并使用SPSS统计软件进行对hs-CRP与基因型间的相关性进行分析。结果 (1)脂联素基因外显子2 Glyl5Gly多态性位点的TG+GG基因型频率及G等位基因频率在T2DM组中明显高于正常对照组(P<0.05)；而各基因型和等位基因频率在糖调节正常一级亲属组和正常对照组中的分布无显著差异(P>0.05)；(2)在T2DM组中，TG+GG基因型CRP水平及腹围高于TT基因型(P<0.05)；在NGT一级亲属组和正常对照组中，TT基因型、TG+GG基因型者各临床和生化指标，以及血清CRP水平相比较，差异无统计学意义(P>0.05)；(3)Logistic分析表明，在T2DM组中，TG+GG基因型与CRP水平具有相关性(P<0.05)；NGT一级亲属组和正常对照组脂联素基因各基因型与CRP水平无相关性(P>0.05)。结论 脂联素基因外显子2Glyl5Gly位点多态性与T2DM患者有相关性，可能对T2DM肥胖和炎症反应有影响；而该基因位点多态性与T2DM患者NGT一级亲属无相关性，并且对NGT一级亲属炎症反应无影响。
Objective To investigate the relationship of adiponectin gene polymorphism and the high sensitive C-reactive protein in type 2 diabetes(T2DM) and normal glucose tolerance(NGT) first degeree relatives of T2DM. Methods (1) We studied 553 subjects from Chongqing Han families which were enrolled . 251 of them were T2DMS, 175 of them were the NGT first degree relatives(FDR) of type 2 diabetes of the families, and 127 of them were the spouses of T2DM family members. The Anthropometry parameter, blood pressure, renal function parameter, oral glucose tolerance test(OGTT), lipid levels, high sensitive C-reactive protein(hsCRP) were examined. IGR were defined according to World Health Organization (WHO) diagnostic criteria. (2) The Gly15Gly variation(T>G) in exon 2 of adiponectin gene was genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay in the subjects. The clinical and biochemical indicators were compared among the genotypes between the groups. The SPSS statistics software was applied to analyse the relations between hs-CRP and the gene polymorphism. Results (1)The distribution of TG+GG genotypes and G allele frequency of adiponectin gene Gly15Gly variation(T>G) in exon 2 were higher in T2DM group than those in the normal control group; There was no obvious distinction of the distribution of genotypes and allele frequency between the normal control group and the NGT FDR group. (2)In T2DM group, the CRP level and abdominal circumference(AC) of TG+GG genotype. were higher than those of TT genotype(P<0.05). In IGR FDR group and normal control group, clinical and biochemical indicators and hs-CRP levels of TG+GG、TT genotype had no significant deviation(P>0.05). (3)Logistic analysis showed that CRP levels were correlated with TG+GG genotype in T2DM group(P<0.05); while in FDR and normal control groups, CRP levels had no correlation with TT and TG+GG genotype(P>0.05). Conclusion Adiponectin gene Gly15Gly variation(T>G) in exon 2 was correlated with CRP in T2DM of the T2DM families. There was no association between the variation and the NGT FDR. The variation of adiponectin gene maybe had influence on the inflammatory reactive and obese in T2DM.

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