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乳化七氟烷预处理对大鼠肝脏缺血再灌注损伤的保护作用
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DOI:
作者:
王莉琴 翟东 蔡玲 陈昼琳 陈淑君 李志高 王超 周新华
Wang Liqin, Zhai Dong, Cai Ling, et al
作者单位:
解放军第85医院麻醉科
Department of Anesthesiology, 85th Hospital of PLA
关键词:
乳化七氟烷 缺血再灌注损伤 肝脏
Emulsified Sevoflurane; Ischemia Reperfusion injury; Liver
摘要:
【摘要】 目的 研究乳化七氟烷预处理对大鼠肝脏缺血再灌注损伤的保护作用。方法 40只健康雄性SD大鼠随机分成4组,分别为假手术组(S组,6只);缺血再灌注组(IR组,10只):肝脏缺血60min,4h后处死;20%脂肪乳预处理组(FAT组,12只):IR前30min经尾静脉泵注20%脂肪乳,速率10ml/kg/h,余同IR组;乳化七氟烷组(SEVO组,12只):IR前30min经尾静脉泵注3.6%(容积比)乳化七氟烷,速率10ml/kg/h,余同IR组。观察各组大鼠肝组织病理改变,测血清ALT、AST,ELISA法测血清IL-1、IL-10和TNF-α。 结果 与IR组和FAT组比较,SEVO组ALT、AST、IL-1和TNF-α明显降低(P<0.05),而IL-10则明显升高 (P<0.05);病理示IR组及FAT组可见小叶中央肝细胞呈空泡样变性并聚集成簇,大量肝细胞胞核浓缩并深染,呈坏死前改变,部分肝细胞坏死,肝血窦及小叶中央重度充血肿胀。SEVO组肝小叶结构尚存,肝细胞轻度水肿,小叶中央个别肝细胞呈坏死前改变,部分肝血窦狭窄;门管区少量炎性细胞浸润,肝细胞损伤程度较IR组轻。结论 乳化七氟烷预处理对大鼠肝脏热缺血再灌注损伤具有保护作用,ALT和AST明显下降,肝细胞损伤减轻,其机制可能与抑制炎性细胞因子生成及促进抗炎细胞因子表达有关。
【Abstract】 Objective To investigate the protection effect of emulsified sevoflurane preconditioning on rat liver ischemia-reperfusion (I/R) injury. Methods Forty male SD rats were randomly divided into four groups, including sham-operation group(S, n=6); ischemia reperfusion group (IR, n=10): receiving 60 mins of hepatic ischemia and 4 hours reperfusion. 20% fat emulsion group(Lipid, n=12): 20% Intralipid was infused through tail vein at the rate of 10ml/kg/h for 30 mins prior to IR. The other procedures were the same as IR group. Emulsified sevoflurane group(Sevo, n=12): 3.6%(v/v) emulsified sevoflurane was infused through tail vein at the rate of 10ml/kg/h for 30 mins prior to IR. The other procedures were the same as IR group. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined by rate method as well as examining the liver pathological change by H-E stain. Tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1), and interleukin-10 (IL-10) were analyzed with enzyme linked immunosorbent assay. Results ALT, AST, TNF-α and IL-1 were markedly decreased in group Sevo than in group IR and FAT (P<0.05), while IL-10 was obviously elevated (P<0.05). Massive neutrophils accumulation and a lot of congestion, vacuolization, and necrosis could be seen in the group IR and FAT. There were less neutrophil accumulation and very limited congestion, vacuolization, and necrosis in the SEVO group. The injury of hepatocytes was ameliorated by emulsified sevoflurane than in group IR and FAT. Conclusion Emulsified sevoflurane preconditioning can reduce the ischemia reperfusion injury of rat liver, evidenced with decreased ALT and AST, together with diminished hepatocyte injury. The protective mechanism may be related with the reduced expression of pro-inflammation cytokines and elevated expression of anti-inflammation cytokine.
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