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地塞米松对大鼠膈肌形态学及力学特性的影响
Effect of chronic dexamethasone treatment on the morphology and contractile property in rat diaphragm
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DOI:
作者:
陈丹
Chen Dan
作者单位:
上海交通大学附属第一人民医院麻醉科
Department of Anesthesiology, First People’s Hospital, School of Medicine, Shanghai Jiaotong University
关键词:
糖皮质激素、类固醇肌病、膈肌、萎缩、纤维类型、收缩特性
glucocorticoid, steroid myopathy, diaphragm, atrophy, fiber type, contractile property
摘要:
目的:探讨慢性应用地塞米松对大鼠膈肌纤维组成、超微结构及收缩功能的影响。方法:成年雄性SD大鼠60只,随机均分为三组,地塞米松组每天腹腔注射地塞米松(0.6mg/kg),生理盐水组注射等体积生理盐水,饮食控制组提供与地塞米松组等量食物且接受等体积生理盐水注射,均注射14天。采用ATP酶染色研究膈肌纤维组成及横截面积,HE染色和透射电镜观察膈肌组织形态及超微结构,并通过建立离体膈肌模型测定收缩功能。结果:与其余两组相比,地塞米松组膈肌Ⅱ型纤维比例和横截面积均显著缩小(P<0.01);可见神经脱髓鞘病变及肌组织横小管扩张;高频刺激下强直收缩张力显著减小(P<0.05),达峰时间和半舒张均明显延长(P<0.05),疲劳指数明显增加(P<0.05)。结论:地塞米松慢性应用,可引起膈肌收缩功能的变化,与激素诱导的快肌向慢肌的转化相关。
Aim: To investigate the influence of chronic dexamethasone (dex) administration on rat diaphragm fiber conformation, ultrastructure and function. Methods: Sixty adult male Sprague-Dawley rats were randomized into three groups (20 animals each). The dex group received a daily intraperitoneal injection of dex (600 μg/kg body mass), the saline group received an equivalent volume of saline (0.9% NaCl) daily, and the food-restricted group (fr) was pair-fed with the dex group and received an equivalent volume of saline, all for 14 days. Diaphragm fiber conformation and cross-sectional area (CSA) were evaluated by adenosine triphosphatase (ATPase) staining; histology and ultrastructure were evaluated by HE staining and transmission electron microscopy; contractile properties were evaluated by in vitro study of diaphragm preparations. Results: Compared with the saline and food restricted groups, the distribution and CSA of type II fibers were decreased in rats exposed to dex (P<0.01). Demyelination and axonal degeneration as well as Swelling transverse tubules were seen. Dex administration led to impaired diaphragm force generation over a wide range of stimulation frequencies, prolonged half-relaxation time as well as time-to-peak tension (P<0.05),and increased fatigue resistance (P<0.05). Conclusion: This study indicates that chronic dex treatment induced alterations in muscle function, and is related to muscle fiber-type transformation.
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